Product Name: Phospho-WNK1 (Thr60) Antibody#4946
Application: Dilutions
Western Blotting: 1:1000
Immunoprecipitation:
Immunohistochemistry:
Peptide ELISA:
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
CAS NO.: 897383-62-9
Product: AST-1306
Specificity Sensitivity: Phospho-WNK1 (Thr60) Antibody detects endogenous levels of WNK1 only when phosphorylated at threonine 60.
Species Reactivity: Human
Source Purification: Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresonding a region surrounding Thr60 of human WNK1. Antibodies are purified by protein A and peptide affinity chromatography.
Background: The WNK [with no lysine (K)] family of serine/threonine kinases is characterized by having a cysteine in place of lysine in subdomain II of its kinase activation domain (1,2). The lysine necessary for phosphoryl transfer is located in an atypical position
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/12181313?dopt=Abstract
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