Product Name: Wee1 Antibody#4936
Application: Dilutions
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Immunohistochemistry:
Peptide ELISA:
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
CAS NO.: 550999-74-1
Product: EVP-6124 (hydrochloride)
Specificity Sensitivity: Wee1 Antibody detects endogenous levels of Wee1 protein independent of phosphorylation.
Species Reactivity: Human, Monkey
Source Purification: Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino-terminus of human Wee1. Antibodies are purified by protein A and peptide affinity chromatography.
Background: Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. The critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14 (1,2). Phosphorylation at Tyr15 and Th
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/25120649?dopt=Abstract
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