Product Name: Phospho-Wee1 (Ser642) (D47G5) Rabbit mAb#4910
Application: Dilutions
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Immunohistochemistry:
Peptide ELISA:
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
CAS NO.: 866206-54-4
Product: PRX-08066
Specificity Sensitivity: Phospho-Wee1 (Ser642) (D47G5) Rabbit mAb detects endogenous levels of wee1 protein only when phosphorylated at Ser642.
Species Reactivity: Human
Source Purification: Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser642 of human wee1.
Background: Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. The critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14 (1,2). Phosphorylation at Tyr15 and Th
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/18669662?dopt=Abstract
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