Product Name: Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb#9664
Application: Dilutions
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Immunohistochemistry: 1:1000
Peptide ELISA:
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
CAS NO.: 945531-77-1
Product: Ezutromid
Specificity Sensitivity: Cleaved Caspase-3 (Asp175) (5A1) Rabbit mAb detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175. This antibody does not recognize full length caspase-3 or other cleaved caspases. No
Species Reactivity: Human, Mouse, Rat, Monkey
Source Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues adjacent to Asp175 of human caspase-3.
Background: Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activ
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/23177618?dopt=Abstract
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