Product Name: Phospho-ATF-2 (Thr71) Antibody#9221
Application: Dilutions
Western Blotting: 1:1000
Immunoprecipitation: 1:250
Immunohistochemistry: 1:50
Peptide ELISA:
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
CAS NO.: 22260-51-1
Product: Bromocriptine (mesylate)
Specificity Sensitivity: Phospho-ATF-2 (Thr71) Antibody detects endogenous levels of ATF-2 only when phosphorylated at threonine 71. This antibody does not cross-react with phosphorylated c-Jun, CREB or other transcription factors. It recognizes both Thr69/Thr71 dually phosphoryl
Species Reactivity: Human, Mouse, Rat, Monkey
Source Purification: Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr71 of human ATF2. Antibodies are purified by protein A and peptide affinity chromatography.
Background: The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressor
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/18433993?dopt=Abstract
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