Product Name: Akt (E17K Mutant Specific) (D1T7P) Rabbit mAb#14702
Application: Dilutions
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Immunohistochemistry:
Peptide ELISA:
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
CAS NO.: 54827-18-8
Product: TMB (monosulfate)
Specificity Sensitivity: Akt (E17K Mutant Specific) (D1T7P) Rabbit mAb recognizes levels of transfected E17K mutant Akt1 and Akt2 protein. This antibody weakly recognizes transfected E17K mutant Akt3.
Species Reactivity: Human
Source Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to E17K mutant sequence of human Akt1 protein.
Background: Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/18413654?dopt=Abstract
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