Pollen surface (AtABCG26) [71]. ABCG was also the largest subfamily of ABC transporters in S.

Pollen surface (AtABCG26) [71]. ABCG was also the largest subfamily of ABC transporters in S. miltiorrhiza, comprised of 46 members (Table 1 and Fig. four). 4 genes (SmABCG40, SmABCG46, SmABCG4, and SmABCG44) had tissue-specific expression profiles within this subfamily, all of which have been very expressed inside the rootsYan et al. BMC Genomics(2021) 22:Page 8 ofFig. three Phylogenetic tree of the ABCC and ABCD/E/F subfamily. Phylogenetic analysis of ABCC (a) and ABCD/E/F (b) proteins of S. miltiorrhiza, Arabidopsis and also other plantsof S. miltiorrhiza (Table 1). Notably, SmABCG4 was the most NTR1 Modulator site hugely expressed gene in the periderm of S. miltiorrhiza roots (Table 1). Offered that tanshinone is synthesized and accumulates in large amounts within the roots of S. miltiorrhiza, specifically inside the periderm tissues [24], it is doable that these four transporters could be related to the transport of tanshinone in S. miltiorrhiza. Phylogenetic analysis revealed that SmABCG4 and SmABCG40 cluster fairly closely with all the ginsenoside transporter Panax ginseng PgPDR3 [72] plus the antifungal terpenoid transporter NpABC1 in Nicotiana plumbaginifolia and NtPDR1 in N. tabacum [73, 74] (Fig. 4). SmABCG46 and SmABCG44 were closely related to AtABCG39 [63] and AtABCG34 [64], which play roles in response to pressure in Arabidopsis. MeJA induced the expressionof SmABCG46 and SmABCG44 at diverse levels, which was homologous to the MeJA induction of AtABCG34 in Arabidopsis (Table 1). An additional full-sized transporter, SmABCG45, possessing the exact same gene structure and abundance as SmABCG46, was also hugely expressed within the roots of S. miltiorrhiza (Further file 1: Figure S1 and Table 1). These five genes of your SmABCG subfamily could be involved in terpenoid transport in S. miltiorrhiza, which could also mediate the pressure responses of this medicinal plant. While it has the identical gene structure as SmABCG46, SmABCG35 was only expressed inside the flowers (Table 1 and More file 1: Figure S1), which suggests that this gene may be involved inside the transport of substances inside the flowers of S. miltiorrhiza.Yan et al. BMC Genomics(2021) 22:Web page 9 ofFig. four Phylogenetic tree of your ABCG subfamily. Phylogenetic evaluation of ABCG proteins of S. miltiorrhiza, Arabidopsis and other plantsSmABCG32 was a full-sized transporter and very expressed in the leaves. Its homologous protein CrTPT2 in Catharanthus roseus is responsible for the transport of catharanthine [75], suggesting that SmABCG32 could possibly be involved inside the transport of secondary metabolites in the leaves of S. miltiorrhiza. Also, six half-sized ABCG transporters were expressed in numerous organs and showed larger expression levels inside the flowers. By way of example, SmABCG12 showed greater expression levels in the flowers in comparison to other tissues. SmABCG12’s homologue, SSTR2 Agonist medchemexpress AtABCG25, participates within the export of abscisic acid [61], indicating that SmABCG12 might be involved within the transport of plant hormones in the flowers of S. miltiorrhiza. SmABCG19 was also very expressed inside the flowers and was homologous to AtABCG11 in Arabisopsis [66] and GhWBC1 in cotton [76], suggesting that SmABCG19 likely played roles inside the transport substances that happen to be connected to the growth and development of S. miltiorrhiza. SmABCG27 and SmABCG28 showed the same expression patterns and had been a lot more extremely expressed within the flowers when compared with the rest of your plant.Both of SmABCG27 and SmABCG28 had been half-sized proteins and had been expressed in all organs exce.