Product Name :
Rabbit anti-Glucocorticoid Receptor Polyclonal Antibody
Synonym :
Glucocorticoid Receptor beta; GCCR; GCR; GCR_HUMAN; glucocorticoid nuclear receptor variant 1; Glucocorticoid receptor beta isoform; GR; GRL; nr3c1; Nuclear receptor subfamily 3 group C member 1
Host :
Rabbit
Species Reactivity:
Human, Mouse, Rat
Specificity :
Glucocorticoid Receptor beta
Predicted Reactivity:
Chicken, Dog, Pig, Cow, Horse, Sheep
Applications :
WB=1:500-2000, Flow-Cyt=1ug/Test
Immunogen:
KLH conjugated synthetic peptide derived from human Glucocorticoid Receptor beta:51-150/777
Concentration :
1mg/ml
Purification :
affinity purified by Protein A
Clonality:
Polyclonal Antibody
Storage Temp.:
Store at -20 ° C for one yearAvoid repeated freeze/that cycles
Research areas :
Cardiovascular Cell biology Signal transduction Zinc finger protein Epigenetics
Background :
Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex’s subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor.
UniProt :
P04150
Additional information:
Product Details FAQ Citations(0) Video Pictures Documents |Overview |Synonym Glucocorticoid Receptor beta; GCCR; GCR; GCR_HUMAN; glucocorticoid nuclear receptor variant 1; Glucocorticoid receptor beta isoform; GR; GRL; nr3c1; Nuclear receptor subfamily 3 group C member 1 |Host Rabbit |Specificity Glucocorticoid Receptor beta |Species Reactivity Human, Mouse, Rat |Predicted Reactivity Chicken, Dog, Pig, Cow, Horse, Sheep |Applications WB=1:500-2000, Flow-Cyt=1ug/Test |Immunogen KLH conjugated synthetic peptide derived from human Glucocorticoid Receptor beta:51-150/777 |Properties |Concentration 1mg/ml |Purification affinity purified by Protein A |Clonality Polyclonal Antibody |Isotype IgG |Storage Temp. Store at -20 ° C for one yearAvoid repeated freeze/that cycles |Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |Research areas Cardiovascular Cell biology Signal transduction Zinc finger protein Epigenetics |Target |Background Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex’s subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor. |Cellular localization Cell nucleus; Cytoplasm; |UniProt P04150 | Sample: A549 (human)Cell Lysate at 40 ugPrimary: Anti-Glucocorticoid Receptor beta(abs122556) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 86 kDObserved band size: 73 kD| Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer at 37°C for 30min; Antibody incubation with (GCR) Polyclonal Antibody, Unconjugated secondary primary antibody at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |Tips:This product is for research use only. Not for use in diagnostic prodcedures.
Rabbit anti-Glucocorticoid Receptor Polyclonal Antibody
Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.Related websites: https://www.medchemexpress.com/antibodies.html
2230911-59-6 custom synthesis 1634620-63-5 Description PMID:29489276 MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com
