Mistry | Cell biologyAdditional informationCompeting interests DR: Reviewing editor, eLife. The otherMistry | Cell biologyAdditional

Mistry | Cell biologyAdditional informationCompeting interests DR: Reviewing editor, eLife. The other
Mistry | Cell biologyAdditional informationCompeting interests DR: Reviewing editor, eLife. The other authors declare that no competing interests exist. FundingFunder Healthcare Analysis Council (MRC) Wellcome Trust British Lung Foundation (BLF) June Hancock Mesothelioma Study Fund Wellcome Trust Diabetes UK Wellcome Trust Grant reference G1002610 084812Z08Z APHD11-4 JH09-2 100140 120004595 CIMR PhD programme Author Stefan J Marciniak David Ron Hanna J Clarke Lucy E Dalton David Ron Vruti Patel Caia S DominicusThe funders had no function in study style, information collection and interpretation, or the choice to submit the function for publication.Author contributions JEC, Performed experiments like generation and use of stables cells for tetracycline-inducible expression of mDia2 fusion proteins, in vivo assessment of eIF2 Bax Compound phosphatase activity by use of eIF2 kinase inhibition and contributed towards the writing on the manuscript., Acquisition of data, Evaluation and interpretation of information, Drafting or revising the article; LED, Performed experiments including GFP-affinity purification of PPP1R15A and mass spectroscopy, deletion mapping for in vivo actin interactions, manipulation of actin polymerisation status by drugs and serum, in vitro eIF2 phosphatase assay, generated steady cells for tetracycline-inducible expression of PPP1R15A, and contributed towards the writing of your manuscript., Acquisition of information, Evaluation and interpretation of information, Drafting or revising the short article; HJC, Performed experiments which includes GFP-affinity purification of PPP1R15A for mass spectroscopy, examined impact of jasplakinolide in eIF2 kinase IL-8 medchemexpress knockout lines, manipulation of actin polymerisation status by drugs and serum, and contributed towards the writing in the manuscript., Acquisition of information, Analysis and interpretation of data, Drafting or revising the short article; EM, Perform affinity purification and deletion mapping experiments involving dPPP1R15, and contributed for the writing from the manuscript., Acquisition of information, Evaluation and interpretation of information, Drafting or revising the report; CSD, Performed experiments which includes GFP-affinity purification of PPP1R15B for mass spectroscopy, and contributed for the writing with the manuscript., Acquisition of data, Evaluation and interpretation of data, Drafting or revising the post; VP, Examined the effect of manipulating actin polymerisation status by drugs and contributed towards the writing with the manuscript., Acquisition of information, Analysis and interpretation of data, Drafting or revising the post; GM, Greg Moorhead synthesised and offered microcysin agarose. The labour-intensive production of this reagent (that is not available commercially) enabled a important experiment. He also contributed his knowledge in phosphatase biology to review and approve the final version in the manuscript., Drafting or revising the write-up, Contributed unpublished crucial data or reagents; DR, Contributed to experimental tactics, made mDia2 constructs and contributed for the writing of your manuscript., Acquisition of information, Evaluation and interpretation of information, Drafting or revising the post; SJM, Conceived and oversaw the study as a complete, found the interaction involving PPP1R15A and actin, and wrote the manuscript., Conception and design and style, Evaluation and interpretation of information, Drafting or revising the post Author ORCIDs David Ron, http:orcid.org0000-0002-3014-5636 Stefan J Marciniak, http:orcid.org0000-0001-8472-
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