Is Gh1 for aqueous two-phase fermentation in two L Figure 3. Comparison of K worth,

Is Gh1 for aqueous two-phase fermentation in two L Figure 3. Comparison of K worth, PF and yield of BLIS from L. lactis Gh1 for aqueous two-phase fermentation in two L bioreactor and Erlenmeyer flask. At the optimum situations of a ten (w/w) PEG2000, 8 (w/w) dextran T500, pH 7 and flask. the optimum situations of a 10 (w/w) (w/w) dextran T500, agitated at 200 rpm at 30 C. (……) ATPS in Erlenmeyer flask, and (___) ATPS in 2 L bioreactor. . ATPS in 2 L bioreactor. ATPS flask,The cell viability of L. lactis Gh1 through homogeneous, and aqueous two-phase fermentation both in an Erlenmeyer flask in addition to a bench-scale bioreactor had been compared (Figure 4). A greater cell viability was obtained from homogeneous fermentation in comparison with that of extractive fermentation in both Erlenmeyer flask and also a bench-scale bioreactor. The presence of polymers (i.e., PEG and dextran) reduced the development price of L. lactis Gh1.Fermentation 2021, 7,Figure 3. Comparison of K worth, PF and yield of BLIS from L. lactis Gh1 for aqueous two-phase fermentation in 2 L bioreactor and Erlenmeyer flask. At the optimum circumstances of a 10 (w/w) PEG2000, eight (w/w) dextran T500, pH 7 and agitated at 200 rpm at 30 . (……) ATPS in Erlenmeyer flask, and (___) ATPS in 2 L bioreactor.14 ofTheThe cell viability of L. lactis Gh1 for the duration of homogeneous,and aqueous two-phase fermencell viability of L. lactis Gh1 in the course of homogeneous, and aqueous two-phase fermentationboth in an Erlenmeyer flask as well as a a bench-scale bioreactor had been compared (Fig- four). tation both in an Erlenmeyer flask and bench-scale bioreactor had been compared (Figure ure A greater PW0787 Data Sheet cellcell viability was obtained from homogeneous fermentation in comparison with of 4). A larger viability was obtained from homogeneous fermentation in comparison with that thatextractive fermentation in bothboth Erlenmeyer flaska bench-scale bioreactor. The presence of extractive fermentation in Erlenmeyer flask and plus a bench-scale bioreactor. The presence of polymers (i.e., PEG and dextran) reduced therate of L. lactisof L. lactis Gh1. the of polymers (i.e., PEG and dextran) decreased the development development price Gh1. Nevertheless, Nonetheless, the sustainable cells growth wasin aqueous two-phasetwo-phase fermentation sustainable cells growth was obtained obtained in aqueous fermentation in bioreactor in bioreactor make the repetitive fermentation scale achievable. probable. make the repetitive fermentation in a big within a large scaleFigure 4. Comparison from the cell viability of L. lactis Gh1 for homogeneous and aqueous two-phase fermentations in an Erlenmeyer flask as well as a 2 L bioreactor. The cells were grown inside the different fermentation CPI-1189 manufacturer environments (Erlenmeyer flask and batch bioreactor) and media, either with or without the need of polymer had been assayed and compared.The growth pattern of L. lactis Gh1 in extractive fermentation exhibited exactly the same pattern as homogeneous culture in each bioreactor and shake flask. The exponential phase was started right after two h to six h of fermentation and also the maximum viability was observed right after eight h though in flask the highest cell number shifted to h-10. Prolonged ATPS-fermentation within the bioreactor preserves the higher concentration of cells, although the cells enter the phase of death each within the flask ATPS and inside the flask homogeneous culture. Maximum cell number of L. lactis Gh1 (1.09 109 CFU/mL) obtained inside the homogeneous culture in flask was about 360 higher as in comparison to both ATPS fermentation in flask (six.87 108 CFU/mL) and in bioreactor (5.43 108 CFU/mL). three.7. Repet.