Nd KSP using siRNA cocktail yields promising results for eradicating hepatocellular
Nd KSP using siRNA cocktail yields promising results for eradicating hepatocellular carcinoma cells, a new direction for liver cancer treatment. Keywords: Vascular endothelial cell growth factor (VEGF), Kinesin spindle protein (KSP), siRNA cocktail, Proliferation, Apoptosis, Hepatocellular carcinomaBackground Primary liver cancer, hepatoblastoma (HB) and hepatocellular carcinoma (HCC), is one of the most common solid tumors, ranking the fifth in most common malignancy worldwide and the second cause of cancer-related deaths. The major therapeutic strategies in solid tumors as well as HCC are excision of the primary tumor, followed by radiotherapy and chemotherapy. However, in some cases, this treatment still leaves some problems such as metastatic reactivation and subsequent tumor recurrence [1]. Recently,* Correspondence: [email protected] 1 Faculty of Biology, University of Science, Vietnam National University, 227 Nguyen Van Cu Street, Ward 4, District 5, Ho Chi Minh City, Vietnam 2 Department of Animal Biotechnology, Institute of Tropical Biology, Vietnam Academy of Science and Technology, 9/621 Xa lo Ha Noi Street, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam Full list of author information is available at the end of the articlefollowing the rapid advances in molecular biology, many new therapeutic strategies, including RNA interference (RNAi) technology for treating liver cancer at genetic level have been developed [2]. RNAi is a specific gene regulatory mechanism in which activation of an intracellular pathway triggered by small-interfering RNA (siRNA) of 21?3 nucleotides (nt), leading to gene silencing through degradation of a homologous target mRNA [3]. The selective and robust effect of RNAi on gene expression makes it become a valuable tool for basic research in biology, and thereby continue to have a major impact on medical science [4]. Another unique advantage of RNAi is that non-druggable protein targets can also be efficiently knocked-down and possibly achieve therapeutic effects [5]. Therefore, RNAi-based therapeutic strategy presents an effective and simple approach in new area of clinical therapy for HCC.?2014 Doan et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28549975 Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, RDX5791MedChemExpress Tenapanor distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.Doan et al. Biological Research 2014, 47:70 http://www.biolres.com/content/47/1/Page 2 ofIt has been known that human cancer is a gene-related disease involving abnormal cell growth. As a new member of the kinesin superfamily of microtubule-based motors, kinesin Eg5, also called kinesin spindle protein (KSP) or KIF11 participates in mitosis, by separating the microtubules that are attached to the two centrosomes, and contributing to the bipolar arrangement of the spindles [6]. Thus, inhibition of KSP may block the formation of bipolar mitotic spindles of mitotic cells, causing cell-cycle arrest, activation of the mitotic checkpoint, induction of apoptosis and eventually, to cell death [5,7]. KSP gene was found to be lowly expressed in normal primary cells, but higher in transformed cells . Its expression was a.
