Product Name: Phospho-Akt (Thr308) (L32A4) Mouse mAb#5106
Application: Dilutions
Western Blotting: 1:1000
Immunoprecipitation:
Immunohistochemistry:
Peptide ELISA:
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
CAS NO.: 17354-14-2
Product: Solvent Blue 35
Specificity Sensitivity: Phospho-Akt (Thr308) (L32A4) Mouse mAb detects endogenous levels of Akt only when phosphorylated at Thr308.
Species Reactivity: Human, Mouse, Rat, Monkey
Source Purification: Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Thr308 of mouse Akt.
Background: Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/20157025?dopt=Abstract
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