Product Name: Phospho-Akt (Thr308) Antibody#9275
Application: Dilutions
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Immunohistochemistry:
Peptide ELISA:
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
CAS NO.: 1429749-41-6
Product: Thiomyristoyl
Specificity Sensitivity: Phospho-Akt (Thr308) Antibody detects endogenous levels of Akt only when phosphorylated at Thr308. This antibody does not detect Akt phosphorylated at other sites or related kinases such as PKC and p70 S6 kinase.
Species Reactivity: Human, Mouse, Rat
Source Purification: Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Thr308 of mouse Akt. Antibodies are purified by protein A and peptide affinity chromatography.
Background: Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/19096978?dopt=Abstract
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