Product Name: Phospho-Akt (Ser473) Antibody#9271
Application: Dilutions
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Immunohistochemistry:
Peptide ELISA:
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
CAS NO.: 1314873-02-3
Product: BPO-27 (racemate)
Specificity Sensitivity: Phospho-Akt (Ser473) Antibody detects endogenous levels of Akt1 only when phosphorylated at Ser473. This antibody also recognizes Akt2 and Akt3 when phosphorylated at the corresponding residues. It does not recognize Akt phosphorylated at other sites, nor
Species Reactivity: Human, Mouse, Rat, Hamster, D. melanogaster, Bovine, Dog, Pig
Source Purification: Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser473 of mouse Akt. Antibodies are purified by protein A and peptide affinity chromatography.
Background: Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/21494640?dopt=Abstract
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