Product Name: Phospho-AMPKα (Thr172) (D4D6D) Rabbit mAb#50081
Application: Dilutions
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Immunohistochemistry: 1:400
Peptide ELISA:
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
CAS NO.: 920509-32-6
Product: MGL-3196
Specificity Sensitivity: Phospho-AMPKα (Thr172) (D4D6D) Rabbit mAb detects endogenous AMPKα only when phosphorylated at threonine 172. The antibody detects both α1 and α2 isoforms of the catalytic subunit, but does not detect the regulatory β or γ subunits.
Species Reactivity: Human, Mouse, Rat
Source Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr172 of human AMPKα protein.
Background: AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis (1). AMPK is a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits,
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/24970395?dopt=Abstract
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