Product Name: Phospho-AMPKα (Thr172) Antibody#2531
Application: Dilutions
Western Blotting: 1:1000
Immunoprecipitation:
Immunohistochemistry:
Peptide ELISA:
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
CAS NO.: 948841-07-4
Product: TB5
Specificity Sensitivity: Phospho-AMPKalpha (Thr172) Antibody detects endogenous AMPKα only when phosphorylated at threonine 172. The antibody detects both α1 and α2 isoforms of the catalytic subunit, but it does not detect the regulatory beta or gamma subunits.
Species Reactivity: Human, Mouse, Rat, Monkey
Source Purification: Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr172 of human AMPKα. Antibodies are purified by protein A and peptide affinity chromatography.
Background: AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis (1). AMPK is a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits,
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/23757406?dopt=Abstract
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