Product Name: Phospho-AMPKα1 (Ser485)/AMPKα2 (Ser491) Antibody#4185
Application: Dilutions
Western Blotting: 1:1000
Immunoprecipitation:
Immunohistochemistry:
Peptide ELISA:
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
CAS NO.: 345303-91-5
Product: AZD9056 (hydrochloride)
Specificity Sensitivity: Phospho-AMPKα1 (Ser485)/AMPKα2 (Ser491) Antibody detects endogenous levels of AMPKα1/α2 only when phosphorylated at serine 485 or serine 491. This antibody does not cross-react with other related proteins.
Species Reactivity: Human, Mouse, Rat, Monkey
Source Purification: Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser491 of human AMPKα2. Antibodies are purified by protein A and peptide affinity chromatography.
Background: AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis (1). AMPK is a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits,
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/22155930?dopt=Abstract
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