Product Name: Phospho-AMPKα1 (Ser485) (45F5) Rabbit mAb#2537
Application: Dilutions
Western Blotting: 1:1000
Immunoprecipitation:
Immunohistochemistry:
Peptide ELISA:
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
CAS NO.: 664334-36-5
Product: BKT140
Specificity Sensitivity: Phospho-AMPKα1 (Ser485) (45F5) Rabbit mAb detects endogenous levels of AMPKα1 only when phosphorylated at Ser485. The antibody does not cross-react with phosphorylated AMPKα2 or other related proteins.
Species Reactivity: Human, Mouse, Rat, Monkey
Source Purification: Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser485 of human AMPKα1.
Background: AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis (1). AMPK is a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits,
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/24042504?dopt=Abstract
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