Product Name: Bub3 (D4F9) Rabbit mAb#8195
Application: Dilutions
Western Blotting: 1:1000
Immunoprecipitation:
Immunohistochemistry:
Peptide ELISA:
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
CAS NO.: 287194-41-6
Product: (+)-DHMEQ
Specificity Sensitivity: Bub3 (D4F9) Rabbit mAb recognizes endogenous levels of total Bub3 protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxy terminus of human Bub3 protein.
Background: The Mitotic Checkpoint Complex (MCC), which contains Bub1, Bub1b, Bub3, Mad2, and Cdc20, controls chromosome segregation and monitors kinetochore-microtubule interactions (1). During mitosis, the MCC complex inhibits the ubiquitin ligase activity of the A
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/24956373?dopt=Abstract
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