Product Name: Bub1b Antibody#4116
Application: Dilutions
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Immunohistochemistry:
Peptide ELISA:
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
CAS NO.: 548-04-9
Product: Hypericin
Specificity Sensitivity: Bub1b Antibody detects endogenous levels of total Bub1b protein.
Species Reactivity: Human
Source Purification: Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acid sequence surrounding Lys370 of human Bub1b. Antibodies are purified by Protein A and peptide affinity chromatography.
Background: The Mitotic Checkpoint Complex (MCC), which contains Bub1, Bub1b, Bub3, Mad2, and Cdc20, controls chromosome segregation and monitors kinetochore-microtubule interactions (1). During mitosis, the MCC complex inhibits the ubiquitin ligase activity of the A
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/24682951?dopt=Abstract
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