Product Name: BrdU (Bu20a) Mouse mAb#5292
Application: Dilutions
Western Blotting:
Immunoprecipitation:
Immunohistochemistry: 1:200
Peptide ELISA:
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
CAS NO.: 56-59-7
Product: Felypressin
Specificity Sensitivity: BrdU (Bu20a) Mouse mAb detects BrdU when incorporated into single stranded DNA. DNA must be denatured for the epitope to be exposed and recognized by the antibody.
Species Reactivity: All Species Expected
Source Purification: Monoclonal antibody is produced by immunizing animals with BrdU conjugated to BSA.
Background: Halogenated nucleotides such as the pyrimidine analog bromodeoxyuridine (BrdU) are useful for labeling nascent DNA in living cells and tissues. BrdU becomes incorporated into replicating DNA in place of thymidine and subsequent immunodetection of BrdU usi
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/23988668?dopt=Abstract
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