Product Name: Akt1 (2H10) Mouse mAb#2967
Application: Dilutions
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Immunohistochemistry:
Peptide ELISA:
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
CAS NO.: 1361224-53-4
Product: AMG-3969
Specificity Sensitivity: The Akt1 isoform specific (2H10) Mouse mAb detects endogenous levels of Akt1, regardless of its phosphorylation state and does not cross-react with Akt 2 and Akt 3.
Species Reactivity: Human, Mouse, Rat
Source Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide. Antibody is purified by protein G affinity chromatography.
Background: Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/18420386?dopt=Abstract
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