We noticed multiple cases when the difference in expression shown in our heat map correlated with previously reported data on deregulation of the gene

Reverse sequence 59-CTGTAGGTGAAGCGGCTGTT-39 59-GACCTCCAGGGCCACCTCGT-39 59-CAGGTAGCGTTTCCCAATGT-39 59-CATCCATGCTGTGGTAATGC-39 59-ATCCACAGCAGCACCTTTCT-39 59-GTTTGGGGGCCGACTGGCTG-39 59-TCAGGAGTGACGGGGCCGAG-39 59-CGCATTGCCGTCCTGGGTGT-39 59-GCTGAGGATGCAGCCGCCAG-39 59-TCTGTGGGTGGGGTGGGACG-39 59-CGCAGGAGCCGTCGCTTCTC-39 59-GCCACCCTTCTGCCGGATGC-39 59-GGCATGACGCTAAAGGTGTT-39 company’s protocol. The pyridinoline concentration was expressed as nmole/mg of dry muscle weight. Statistical analyses Each animal was considered an experimental unit. Data were analyzed as a complete randomized design using general linear model of SAS. No difference was observed in data from two age groups and, thus, these data were combined. Differences in mean values were compared by Tukey’s multiple comparison test, and means 6 SE was reported. Statistical significance was considered as P,0.05. Real-time quantitative PCR Total mRNA was extracted from muscle using TRI reagent and reverse transcribed into cDNA by using a kit. RT-PCR was performed using an iQ5 RT-PCR detection system. A SYBR Green RT-PCR kit from Bio-Rad Laboratories was used together with the Ovis aries primers listed in Author Contributions Conceived and designed the MedChemExpress UPF 1069 experiments: MD SPF PWN. Performed the experiments: YH JZ XY MZ NL RJM. Analyzed the data: YH MZ. Wrote the paper: YH PWN MD. 7 February 2012 | Volume 7 | Issue 2 | e31691 Fibrogenesis in Offspring Muscle of Obese Dams 18. Bispham J, Gopalakrishnan GS, Dandrea J, Wilson V, Budge H, et al. Maternal endocrine adaptation throughout pregnancy to nutritional manipulation: consequences for maternal plasma leptin and cortisol and the programming of fetal adipose tissue development. Endocrinology 144: 35753585. 19. Anthony RV, Scheaffer AN, Wright CD, Regnault TR Ruminant models of prenatal growth restriction. Reprod Suppl 61: 183194. 20. Das UG, Schroeder RE, Hay WW, Jr., Devaskar SU Time-dependent and tissue-specific effects of circulating glucose on fetal ovine glucose transporters. Am J Physiol 276: R809817. 21. Hay WW, Jr., Sparks JW, Wilkening RB, Battaglia FC, Meschia G Fetal glucose uptake and utilization as functions of maternal glucose concentration. Am J Physiol 246: E237242. 22. Muhlhausler BS, Duffield JA, McMillen IC Increased maternal nutrition stimulates peroxisome proliferator activated receptor-gamma, adiponectin, and leptin messenger ribonucleic acid expression in adipose tissue before birth. Endocrinology 148: 878885. 23. Sebert SP, Hyatt MA, “26785144 Chan LL, Patel N, Bell RC, et al. Maternal nutrient restriction between early and midgestation and its impact upon appetite regulation after juvenile obesity. Endocrinology 150: 634641. 24. Lee RC, Wang ZM, Heymsfield SB Skeletal muscle mass and aging: regional and whole-body measurement methods. Can J Appl Physiol 26: 102122. 25. Tong JF, Yan X, Zhu MJ, Ford SP, Nathanielsz PW, et al. Maternal obesity downregulates myogenesis and beta-catenin signaling in fetal skeletal muscle. Am J Physiol Endocrinol Metab 296: “ 23977191 E917924. 26. Nissen PM, Danielsen VO, Jorgensen PF, Oksbjerg N Increased maternal nutrition of sows has no beneficial effects on muscle fiber number or postnatal growth and has no impact on the meat quality of the offspring. J Anim Sci 81: 30183027. 27. Greenwood PL, Hunt AS, Hermanson JW, Bell AW Effects of birth weight and postnatal nutrition on neonatal sheep: II. Skeletal muscle growth and development. J Anim Sci 78: 5061. 28. Lahoute C, Sotiropoulos A, Favier M, Guillet-Deniau