The MEK1 p.D67N mutant is activating as demonstrated by enhanced ERK phosphorylation as when compared with empty vector and wildtype MEK1, two controls which don’t activate ERK

escine and ornithine The finding in each subunit of the crystals of PTC-PAPU and PTC-PALO of very elongated masses of non-protein electron density that fit extended PAPU and PALO molecules, have clarified how can PTC discriminate between ornithine and putrescine. The active centers of PTC and OTC are highly similar. One significant difference is the February 2012 | Volume 7 | Issue 2 | e31528 Putrescine Transcarbamylase Structure 10 February 2012 | Volume 7 | Issue 2 | e31528 Putrescine Transcarbamylase Structure the SMG loop, including the replacement of the SMG signature of OTC by the PTC signature 227DVWYGLY233. The influence of this last loop in the selectivity of PTC for putrescine is illustrated by the interactions with putrescine of Y233. In the narrow and mainly hydrophobic sheath that encircles the putrescine moiety of PAPU, the phenolic ring of Y233 sits flat as a tile at the site that would be occupied by the aCOO2 of ornithine, hampering ornithine but not putrescine binding. This phenolic ring is stabilized in such position by hydrophobic contacts with nearby residues and with the hydrocarbon chain of putrescine, as well as by hydrogen binding of its O atom with the side-chain N atom of Q79 from the 80-loop of an adjacent subunit. In contrast, in the PTC-PALO complex the phenolic group of Y233 is far from the ornithine and it interacts extensively with the carbon chain of Q50, leaving widely exposed the ornithine moiety of PALO. This loss of extensive contacts with the hydrocarbon chain of the bound ornithine should strongly decrease the stability of the enzyme-ornithine complex and thus the affinity for ornithine, as reflected in the much larger Km value of PTC for ornithine than for putrescine. A change in the position of Y233 in the PTC-PALO complex may also account for the decreased kcat of PTC for ornithine. In this complex the hydrogen bond between the Y233 phenolic O atom and Q79 is lost and the position of Q79 and of the 80loop is changed. Q79 is crucial for formation by the 80 and 230 loops of a roof over the active center that buries the bound PAPU, and that must be opened and closed in each catalytic cycle to allow substrate access and product release. The roof opening an closing cycle and the catalytic cycle may be synchronized by the hydrogen binding of this N atom of Q79 with an O atom of the Tetrabenazine (Racemate) citations phosphonate moiety of PAPU and thus of CP. Actually, this hydrogen bond links one subunit with the substrate bound to another subunit, supporting the view that in PTC, as in other transcarbamylases, two subunits are needed for building the active center. In any case, the roof over bound PAPU involves the cooperation of both subunits. Although the side chain O atom of Q79 is hydrogen-bonded to one N atom of the imidazole ring of H83 belonging to the 80-loop, the other N atom of this imidazole is bound to the side-chain carboxylate of E236, of the 230-loop of the subunit hosting the active center. The breaking of the roof may be deleterious for “ 23977191 enzyme activity if catalysis requires confinement of the substrates under this closed roof. Indeed, the abolition in the PTC-PALO complex of the hydrogen bond between the phenolic O atom and Q79 also results in the secondary loss of the “ 25331948 hydrogen bond linking Q79 and H83, tearing open at its center the roof over the active center, clearing the way to allow the escape of ornithine. Actually, of the six PTC subunits in the asymmetric unit of the PTC-PALO complex, the 80-loop is not visib