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Sections had been eventually counterstained with DAPI (2 g/mL Invitrogen) and coverslipped working with Aqua-Poly/Mount (Polysciences)

Cells had been last but not least counterstained with 4′,6-Diamidino-two-Phenylindole, Dihydrochloride (DAPI, two g/mL...
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Although EndoC-H1 cells responded to glucose by depolarizing, we unsuccessful to detect oscillations in individual cells, which were apparent in subpopulations of INS-1 832/13 cells

INS-1 832/thirteen cells also showed a higher response to pyruvate (1.6-fold) compared to EndoC-H1...